NSF Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: NSF Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-612-401-E01
• Supplier Catalog Number: 612-401-E01
• Alternative Catalog Number: ROC-612-401-E01
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Anti-NSF Antibody was produced by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region.
• Conjugation: Unconjugated
• Alternative Names: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF

Product Properties

• Clonality: Polyclonal
• NCBI: 60355
• UniProt: Q9QUL6
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 1:1000
• Application Notes: Anti-NSF Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to the NSF protein in the appropriate cell lysate or extract.

Images

Western Blot of Rabbit anti-NSF (N-ethylmaleimide sensitive fusion protein) antibody. Lane 1: rat caudate lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: NSF antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 75 kDa for NSF. Other band(s): none.