Dynamin phospho S774 Antibody, Sheep, Polyclonal

Produktübersicht

• Artikelname: Dynamin phospho S774 Antibody, Sheep, Polyclonal
• Artikelnummer: ROC-612-601-D32
• Hersteller Artikelnummer: 612-601-D32
• Alternativnummer: ROC-612-601-D32
• Hersteller: Rockland Immunochemicals
• Wirt: Sheep
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Rat
• Immunogen: Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
• Konjugation: Unconjugated
• Alternative Synonym: sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100, Dnm, Dnm1

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 140694
• UniProt: P21575
• Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Formulierung: Liquid
• Target-Kategorie: Rat
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, IHC: 1:1000, IF Microscopy: 1:1000, WB: 1:1000
• Anwendungsbeschreibung: Anti-Dynamin pS774 Antibody is tested for use in Western Blotting and ICC and suitable in IHC and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protei

Bilder

Western Blot of Anti-Dynamin pS774 (Sheep) Antibody. Lane 1: rat hippocampal lysate stimulated with forskolin. Lane 2: rat hippocampal lysate stimulated with forskolin incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: Dynamin pS774 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~95kDa/~95kDa for dynamin phosphorylated at Ser774.