Dynamin phospho S774 Antibody, Sheep, Polyclonal

Product Overview

• Article Name: Dynamin phospho S774 Antibody, Sheep, Polyclonal
• Biozol Catalog Number: ROC-612-601-D32
• Supplier Catalog Number: 612-601-D32
• Alternative Catalog Number: ROC-612-601-D32
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Anti-Dynamin pS774 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: sheep anti-Dynamin pS774 Antibody, Dynamin-1, B-dynamin, D100, Dnm, Dnm1

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 140694
• UniProt: P21575
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, IHC: 1:1000, IF Microscopy: 1:1000, WB: 1:1000
• Application Notes: Anti-Dynamin pS774 Antibody is tested for use in Western Blotting and ICC and suitable in IHC and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protei

Images

Western Blot of Anti-Dynamin pS774 (Sheep) Antibody. Lane 1: rat hippocampal lysate stimulated with forskolin. Lane 2: rat hippocampal lysate stimulated with forskolin incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: Dynamin pS774 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~95kDa/~95kDa for dynamin phosphorylated at Ser774.