Dynamin phospho S778 Antibody, Sheep, Polyclonal

Artikelnummer: ROC-612-601-D33
Artikelname: Dynamin phospho S778 Antibody, Sheep, Polyclonal
Artikelnummer: ROC-612-601-D33
Hersteller Artikelnummer: 612-601-D33
Alternativnummer: ROC-612-601-D33
Hersteller: Rockland Immunochemicals
Wirt: Sheep
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Rat
Immunogen: Anti-Dynamin pS778 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH.
Konjugation: Unconjugated
Alternative Synonym: sheep anti-Dynamin pS778 Antibody, Dynamin-1, B-dynamin, D100, Dnm1, Dnm
Klonalität: Polyclonal
Konzentration: Titrated value sufficient to run approximately 10 mini blots.
NCBI: 140694
UniProt: P21575
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Rat
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:10,000, WB: 1:1000
Anwendungsbeschreibung: Anti-Dynamin pS778 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser778 i
Western Blot of Anti-Dynamin pS778 (Sheep) Antibody . Lane 1: rat hippocampal lysate stimulated with forskolin. Lane 2: rat hippocampal lysate stimulated with forskolin incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: Dynamin pS778 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~95kDa/~95kDa for dynamin phosphorylated at Ser778 . Other band(s): none.