Dynamin phospho S778 Antibody, Sheep, Polyclonal

Product Overview

• Article Name: Dynamin phospho S778 Antibody, Sheep, Polyclonal
• Biozol Catalog Number: ROC-612-601-D33
• Supplier Catalog Number: 612-601-D33
• Alternative Catalog Number: ROC-612-601-D33
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Anti-Dynamin pS778 Antibody was produced in sheep by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser778 conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: sheep anti-Dynamin pS778 Antibody, Dynamin-1, B-dynamin, D100, Dnm1, Dnm

Product Properties

• Clonality: Polyclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• NCBI: 140694
• UniProt: P21575
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Rat
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1000
• Application Notes: Anti-Dynamin pS778 Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 95 kDa in size corresponding to dynamin protein phosphorylated at Ser778 i

Images

Western Blot of Anti-Dynamin pS778 (Sheep) Antibody . Lane 1: rat hippocampal lysate stimulated with forskolin. Lane 2: rat hippocampal lysate stimulated with forskolin incubated in lambda-Ptase (1200 units for 30 min). Load: 10 µg per lane. Primary antibody: Dynamin pS778 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~95kDa/~95kDa for dynamin phosphorylated at Ser778 . Other band(s): none.