Goat F(ab)2 anti-Human IgM (µ)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Artikelnummer: ROC-709-1531
Artikelname: Goat F(ab)2 anti-Human IgM (µ)-Alk. Phos., MinX none, ALP, Polyclonal , AP
Artikelnummer: ROC-709-1531
Hersteller Artikelnummer: 709-1531
Alternativnummer: ROC-709-1531
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Human
Immunogen: Human IgM Fc5µ fragment
Konjugation: Alk. Phos.
Alternative Synonym: Goat F(ab)2 Anti-Human IgM Fc5µ Antibody Alkaline Phosphatase Conjugation, Goat Fab2 Anti-Human IgM Fc5µ alk phos Conjugated Antibody
Format: F(ab')2
Spezifität: IgM (µ)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:7,500 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:5,000
Anwendungsbeschreibung: F(ab)2 Anti-Human IgM Fc5µ Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. F(ab)2 Anti-Hu
ELISA Results of F(ab)2 Anti-Human IgM Fc5µ (Goat) Antibody Alkaline Phosphatase Conjugated. Each well was coated in duplicate with 1.0 µg of Human IgM Fc5µ (p/n 009-0131). The working dilution is 15600. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate p/n TMBE-1000.