Goat F(ab)2 anti-Human IgM (µ)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat F(ab)2 anti-Human IgM (µ)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-709-1531
• Supplier Catalog Number: 709-1531
• Alternative Catalog Number: ROC-709-1531
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgM Fc5µ fragment
• Conjugation: Alk. Phos.
• Alternative Names: Goat F(ab)2 Anti-Human IgM Fc5µ Antibody Alkaline Phosphatase Conjugation, Goat Fab2 Anti-Human IgM Fc5µ alk phos Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:7,500 - 1:30,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:5,000
• Application Notes: F(ab)2 Anti-Human IgM Fc5µ Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. F(ab)2 Anti-Hu

Images

ELISA Results of F(ab)2 Anti-Human IgM Fc5µ (Goat) Antibody Alkaline Phosphatase Conjugated. Each well was coated in duplicate with 1.0 µg of Human IgM Fc5µ (p/n 009-0131). The working dilution is 15600. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate p/n TMBE-1000.