Goat F(ab)2 anti-Human IgG (Fc)-Biotin, MinX Bo,Ho,Ms,Rt, Polyclonal

Artikelnummer: ROC-709-1617
Artikelname: Goat F(ab)2 anti-Human IgG (Fc)-Biotin, MinX Bo,Ho,Ms,Rt, Polyclonal
Artikelnummer: ROC-709-1617
Hersteller Artikelnummer: 709-1617
Alternativnummer: ROC-709-1617
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Human
Immunogen: Human IgG F(c) fragment
Konjugation: Biotin
Alternative Synonym: Goat F(ab)2 Anti-Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed, Goat Fab2 Anti-Human IgG Fc Fragment Biotin Conjugated Antibody, Goat F(ab)2 Anti-Human IgG F(c) Fragment Biotin Conjugated Antibody
Format: F(ab')2
Spezifität: IgG (Fc)
Minimale Kreuzreaktivität (MinX): Bovine,Equine,Mouse,Rat
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Human
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:125,000 - 1:175,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: F(ab)2 Anti-Human IgG F(c) Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6
Western Blot of Goat anti-F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed Lane 1: Human Fc Load: 100 ng per lane Primary Antibody: F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed Size: 28 kDa/28 kDa