Goat F(ab)2 anti-Human IgG (Fc)-Biotin, MinX Bo,Ho,Ms,Rt, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Human IgG (Fc)-Biotin, MinX Bo,Ho,Ms,Rt, Polyclonal
• Biozol Catalog Number: ROC-709-1617
• Supplier Catalog Number: 709-1617
• Alternative Catalog Number: ROC-709-1617
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Human
• Immunogen: Human IgG F(c) fragment
• Conjugation: Biotin
• Alternative Names: Goat F(ab)2 Anti-Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed, Goat Fab2 Anti-Human IgG Fc Fragment Biotin Conjugated Antibody, Goat F(ab)2 Anti-Human IgG F(c) Fragment Biotin Conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): Bovine,Equine,Mouse,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:125,000 - 1:175,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Human IgG F(c) Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6

Images

Western Blot of Goat anti-F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed Lane 1: Human Fc Load: 100 ng per lane Primary Antibody: F(ab)2 Human IgG F(c) Antibody Biotin Conjugated Pre-Adsorbed at 1:1000 for 60 min RT Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed Size: 28 kDa/28 kDa