0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
Anwendungsbeschreibung:
Human F(ab)2 IgG (H&L) phycoerythrin conjugated antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, ab
Dot Blot results of Goat F(ab)2 Anti-Human IgG Antibody Phycoerythrin Conjugated. Dots are Human IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat F(ab)2 Anti-Human IgG Antibody RPE at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.
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