Goat F(ab)2 anti-Human IgG (H+L)-RPE, MinX Bo,Ho,Ms,Rt, Polyclonal

Artikelnummer: ROC-709-1816
Artikelname: Goat F(ab)2 anti-Human IgG (H+L)-RPE, MinX Bo,Ho,Ms,Rt, Polyclonal
Artikelnummer: ROC-709-1816
Hersteller Artikelnummer: 709-1816
Alternativnummer: ROC-709-1816
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: FACS,IF
Spezies Reaktivität: Human
Immunogen: Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat.
Konjugation: RPE
Alternative Synonym: Goat F(ab)2 Anti-Human IgG Phycoerythrin Conjugated Antibody Pre-Adsorbed, Goat F(ab)2 Anti-Human IgG Antibody PE Conjugation
Format: F(ab')2
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): Bovine,Equine,Mouse,Rat
Klonalität: Polyclonal
Konzentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Human
Antibody Type: Secondary Antibody
Application Verdünnung: Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
Anwendungsbeschreibung: Human F(ab)2 IgG (H&L) phycoerythrin conjugated antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, ab
Dot Blot results of Goat F(ab)2 Anti-Human IgG Antibody Phycoerythrin Conjugated. Dots are Human IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat F(ab)2 Anti-Human IgG Antibody RPE at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.