Goat F(ab)2 anti-Human IgG (H+L)-RPE, MinX Bo,Ho,Ms,Rt, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Human IgG (H+L)-RPE, MinX Bo,Ho,Ms,Rt, Polyclonal
• Biozol Catalog Number: ROC-709-1816
• Supplier Catalog Number: 709-1816
• Alternative Catalog Number: ROC-709-1816
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: FACS,IF
• Species Reactivity: Human
• Immunogen: Anti-Human IgG (H&L) was produced by repeated immunization with human IgG whole molecule in goat.
• Conjugation: RPE
• Alternative Names: Goat F(ab)2 Anti-Human IgG Phycoerythrin Conjugated Antibody Pre-Adsorbed, Goat F(ab)2 Anti-Human IgG Antibody PE Conjugation
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Equine,Mouse,Rat

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
• Application Notes: Human F(ab)2 IgG (H&L) phycoerythrin conjugated antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, ab

Images

Dot Blot results of Goat F(ab)2 Anti-Human IgG Antibody Phycoerythrin Conjugated. Dots are Human IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat F(ab)2 Anti-Human IgG Antibody RPE at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Rhodamine filter.