Goat F(ab)2 anti-Mouse IgG (H+L)-unconj., MinX none

Artikelnummer: ROC-710-1102
Artikelname: Goat F(ab)2 anti-Mouse IgG (H+L)-unconj., MinX none
Artikelnummer: ROC-710-1102
Hersteller Artikelnummer: 710-1102
Alternativnummer: ROC-710-1102
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Mouse
Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Konjugation: Unconjugated
Alternative Synonym: Goat F(ab)2 Anti-Mouse IgG Antibody, Goat Fab2 Anti-Mouse IgG Antibody
Format: F(ab')2
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Mouse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: F(ab)2 Mouse IgG (H&L) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, ab
Dot Blot Results of F(ab)2 Goat Anti-Mouse IgG Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab)2 Goat Anti-Mouse IgG at 1.0µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP [p/n 605-703-125] at 1µg/mL for 30 mins at RT. Block: BlockOut Buffer [p/n MB-073] at RT for 30 mins. Exposure: 1 sec.