F(ab)2 Mouse IgG (H&L) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, ab
Dot Blot Results of F(ab)2 Goat Anti-Mouse IgG Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab)2 Goat Anti-Mouse IgG at 1.0µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP [p/n 605-703-125] at 1µg/mL for 30 mins at RT. Block: BlockOut Buffer [p/n MB-073] at RT for 30 mins. Exposure: 1 sec.
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