Goat F(ab)2 anti-Mouse IgG (H+L)-unconj., MinX none

Catalog Number: ROC-710-1102
Article Name: Goat F(ab)2 anti-Mouse IgG (H+L)-unconj., MinX none
Biozol Catalog Number: ROC-710-1102
Supplier Catalog Number: 710-1102
Alternative Catalog Number: ROC-710-1102
Manufacturer: Rockland Immunochemicals
Host: Goat
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Mouse
Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in goat.
Conjugation: Unconjugated
Alternative Names: Goat F(ab)2 Anti-Mouse IgG Antibody, Goat Fab2 Anti-Mouse IgG Antibody
Format: F(ab')2
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Mouse
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application Notes: F(ab)2 Mouse IgG (H&L) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, ab
Dot Blot Results of F(ab)2 Goat Anti-Mouse IgG Antibody. Mouse IgG [p/n 010-0102] (1) 100ng, (2) 33.33ng, (3) 11.11ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: F(ab)2 Goat Anti-Mouse IgG at 1.0µg/mL for 1hr at RT. Secondary Antibody: Donkey Anti-Goat IgG HRP [p/n 605-703-125] at 1µg/mL for 30 mins at RT. Block: BlockOut Buffer [p/n MB-073] at RT for 30 mins. Exposure: 1 sec.