Goat F(ab)2 anti-Mouse IgG (H+L)-Biotin, MinX Bo,Ho,Hu,Rb,Rt,Sh, Polyclonal

Artikelnummer: ROC-710-1632
Artikelname: Goat F(ab)2 anti-Mouse IgG (H+L)-Biotin, MinX Bo,Ho,Hu,Rb,Rt,Sh, Polyclonal
Artikelnummer: ROC-710-1632
Hersteller Artikelnummer: 710-1632
Alternativnummer: ROC-710-1632
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Mouse
Immunogen: Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Konjugation: Biotin
Alternative Synonym: Goat F(ab)2 Anti-Mouse IgG Antibody biotin Conjugation, Goat Fab2 Anti-Mouse IgG biotin conjugated Antibody
Format: F(ab')2
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): Bovine,Equine,Human,Rabbit,Rat,Sheep
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Mouse
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: F(ab)2 Anti-Mouse IgG (H&L) (GOAT) Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) me
ELISA Results of Goat F(ab)2 Anti-Mouse IgG Antibody Biotin Min X Bv Hs Hu Rb Rt Sh Serum Proteins. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The working dilution is 127,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated and TMB substrate p/n TMBE-1000.