Goat F(ab)2 anti-Mouse IgG (H+L)-Biotin, MinX Bo,Ho,Hu,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Goat F(ab)2 anti-Mouse IgG (H+L)-Biotin, MinX Bo,Ho,Hu,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-710-1632
• Supplier Catalog Number: 710-1632
• Alternative Catalog Number: ROC-710-1632
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
• Conjugation: Biotin
• Alternative Names: Goat F(ab)2 Anti-Mouse IgG Antibody biotin Conjugation, Goat Fab2 Anti-Mouse IgG biotin conjugated Antibody
• Format: F(ab')2
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Equine,Human,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: F(ab)2 Anti-Mouse IgG (H&L) (GOAT) Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) me

Images

ELISA Results of Goat F(ab)2 Anti-Mouse IgG Antibody Biotin Min X Bv Hs Hu Rb Rt Sh Serum Proteins. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The working dilution is 127,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated and TMB substrate p/n TMBE-1000.