Anti-Mitochondria antibody is tested for use in Western Blotting, IHC and IF. Expect ~60kDa in WB. Anti-Anti-Mitochondria antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraform
Western Blot of Anti-Mitochondria (Mouse) Antibody. Lane 1: HeLa lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Mitochondria antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 60kDa/~60kDa for mitochondrial protein. Other band(s): none.
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