Mitochondria Antibody, IgG1, Clone: [113-1], Mouse, Monoclonal

Product Overview

• Article Name: Mitochondria Antibody, IgG1, Clone: [113-1], Mouse, Monoclonal
• Biozol Catalog Number: ROC-909-301-D79
• Supplier Catalog Number: 909-301-D79
• Alternative Catalog Number: ROC-909-301-D79
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Anti-Mitochondria Monoclonal Antibody was produced by repeated immunizations with a human cell homogenate.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-Mitochondria Antibody, Human cell marker antibody, xenographic model research antibodies, anti-mitochondria antibody produced in mouse

Product Properties

• Clonality: Monoclonal
• Concentration: Titrated value sufficient to run approximately 10 mini blots.
• Clone Designation: [113-1]
• Isotype: IgG1
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:40,000, IHC: 1:800, IF Microscopy: 1:100 - 1:1,000, WB: 1:200 - 1:2,000
• Application Notes: Anti-Mitochondria antibody is tested for use in Western Blotting, IHC and IF. Expect ~60kDa in WB. Anti-Anti-Mitochondria antibody works on acetone fixed and 2% paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2% paraform

Images

Western Blot of Anti-Mitochondria (Mouse) Antibody. Lane 1: HeLa lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Mitochondria antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~ 60kDa/~60kDa for mitochondrial protein. Other band(s): none.