Protein G Peroxidase Conjugated, HRP

Artikelnummer: ROC-PG00-03-0500
Artikelname: Protein G Peroxidase Conjugated, HRP
Artikelnummer: ROC-PG00-03-0500
Hersteller Artikelnummer: PG00-03-0500
Alternativnummer: ROC-PG00-03-0500
Hersteller: Rockland Immunochemicals
Kategorie: Proteine/Peptide
Applikation: DOT, ELISA
Konjugation: HRP
Alternative Synonym: ProG, Streptococcus G protein, Protein G HRP, peroxidase, Horseradish peroxidase protein G
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 19909
UniProt: P19909
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Application Verdünnung: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
Anwendungsbeschreibung: Protein G Peroxidase has been tested by ELISA and dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary
Dot Blot of Protein G Peroxidase Conjugated. Antigen 1: Human IgG. Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: 200ng followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Protein G Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.