Protein G Peroxidase Conjugated, HRP

Product Overview

• Article Name: Protein G Peroxidase Conjugated, HRP
• Biozol Catalog Number: ROC-PG00-03-0500
• Supplier Catalog Number: PG00-03-0500
• Alternative Catalog Number: ROC-PG00-03-0500
• Manufacturer: Rockland Immunochemicals
• Category: Proteine/Peptide
• Application: DOT, ELISA
• Conjugation: HRP
• Alternative Names: ProG, Streptococcus G protein, Protein G HRP, peroxidase, Horseradish peroxidase protein G

Product Properties

• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 19909
• UniProt: P19909
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:40,000
• Application Notes: Protein G Peroxidase has been tested by ELISA and dot blot and is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary

Images

Dot Blot of Protein G Peroxidase Conjugated. Antigen 1: Human IgG. Antigen 2: Rat IgG. Antigen 3: Dog IgG. Load: 200ng followed by a 3-fold serial dilution. Primary antibody: none. Secondary antibody: Protein G Peroxidase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.