HeLa Whole Cell Lysate Doxorubicin Stimulated, HeLa WCL Doxorubicin Stimulated, HeLa Doxorubicin Stimulated Whole Cell Lysate, HeLa Doxorubicin Stimulated WCL
Konzentration:
1.0 mg/mL by BCA assay
Puffer:
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
Quelle:
Human
Formulierung:
Liquid (sterile filtered)
Application Verdünnung:
WB: User Optimized
Anwendungsbeschreibung:
Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been
SDS-PAGE results of Doxorubicin Stimulated HeLa Whole Cell Lysate. Lane 1: HeLa Whole Cell Lysate (p/n W09-001-364) [10µg] Reduced. Lane 2: HeLa Whole Cell Lysate-Doxorubicin Stimulated (W09-001-A79) [10µg] Reduced. Lane 3: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 4: HeLa Whole Cell Lysate (p/n W09-001-364) [10µg] Non-Reduced. Lane 5: HeLa Whole Cell Lysate-Doxorubicin Stimulated (W09-001-A79) [10µg] Non-Reduced. 4-20% Gel, Coomassie Stained.
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