HeLa Whole Cell Lysate Doxorubicin Stimulated

Product Overview

• Article Name: HeLa Whole Cell Lysate Doxorubicin Stimulated
• Biozol Catalog Number: ROC-W09-001-A79
• Supplier Catalog Number: W09-001-A79
• Alternative Catalog Number: ROC-W09-001-A79
• Manufacturer: Rockland Immunochemicals
• Category: Zellen/Zellkultur
• Application: SDS-PAGE
• Conjugation: Unconjugated
• Alternative Names: HeLa Whole Cell Lysate Doxorubicin Stimulated, HeLa WCL Doxorubicin Stimulated, HeLa Doxorubicin Stimulated Whole Cell Lysate, HeLa Doxorubicin Stimulated WCL

Product Properties

• Concentration: 1.0 mg/mL by BCA assay
• Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8)
• Source: Human
• Form: Liquid (sterile filtered)

Application Notes

• Application Dilute: WB: User Optimized
• Application Notes: Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been

Images

SDS-PAGE results of Doxorubicin Stimulated HeLa Whole Cell Lysate. Lane 1: HeLa Whole Cell Lysate (p/n W09-001-364) [10µg] Reduced. Lane 2: HeLa Whole Cell Lysate-Doxorubicin Stimulated (W09-001-A79) [10µg] Reduced. Lane 3: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 4: HeLa Whole Cell Lysate (p/n W09-001-364) [10µg] Non-Reduced. Lane 5: HeLa Whole Cell Lysate-Doxorubicin Stimulated (W09-001-A79) [10µg] Non-Reduced. 4-20% Gel, Coomassie Stained.