Terminal Transferase, Calf (EC 2.7.7.31) is a template-independent polymerase that catalyzes the addition of deoxynucleotides to the 3 hydroxyl ends of DNA molecules. It can utilize double-stranded or single-stranded DNA with protruding, recessed, or blunt ends as substrates. Terminal Transferase, Calf (EC 2.7.7.31) lacks 5 or 3 exonuclease activity. The presence of Co2+ in the reaction can enhance the efficiency of end addition.
Target-Kategorie:
Endogenous Metabolite
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