Integrin beta 4 (CD104), IgG2, Clone: [15H75], Rat, Monoclonal

Produktübersicht

• Artikelname: Integrin beta 4 (CD104), IgG2, Clone: [15H75], Rat, Monoclonal
• Artikelnummer: USB-299164
• Hersteller Artikelnummer: 299164
• Alternativnummer: USB-299164-50
• Hersteller: US Biological
• Wirt: Rat
• Kategorie: Antikörper
• Applikation: IHC, WB
• Immunogen: Recombinant protein corresponding to mouse Integrin beta 4.

Produktbeschreibung

Integrin beta 4, also known as CD104, is a 200kD type I transmembrane (TM) glycoprotein that associates only with Integrin alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22aa TM segment and an unusually long 1073aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Immunohistochemistry(Frozen): 1:100-200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [15H75]
• Isotyp: IgG2
• Reinheit: Purified by Protein G affinity chromatography from culture supernatant.
• Formulierung: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.