Integrin beta 4 (CD104), IgG2, Clone: [15H75], Rat, Monoclonal
Biozol Catalog Number:
USB-299164
Supplier Catalog Number:
299164
Alternative Catalog Number:
USB-299164-50
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
IHC, WB
Immunogen:
Recombinant protein corresponding to mouse Integrin beta 4.
Integrin beta 4, also known as CD104, is a 200kD type I transmembrane (TM) glycoprotein that associates only with Integrin alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22aa TM segment and an unusually long 1073aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-2000 Immunohistochemistry(Frozen): 1:100-200 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clonality:
Monoclonal
Clone Designation:
[15H75]
Isotype:
IgG2
Purity:
Purified by Protein G affinity chromatography from culture supernatant.
Form:
Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile PBS.
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