Aprataxin is a member of the histidine triad (HIT) superfamily, some of which have nucleotide- binding and diadenosine polyphosphate hydrolase activities. Aprataxin may play a role in single- stranded DNA repair. Mutations in the gene have been associated with neurological disorder known as ataxia oculomotor apraxia-1. Recombinant protein corresponding to aa1-168 from human Aprataxin, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~23.9kD (205aa) Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWAGSMQD PKMQVYKDEQ VVVIKDKYPK ARYHWLVLPW TSISSLKAVA REHLELLKHM HTVGEKVIVD FAGSSKLRFR LGYHAIPSMS HVHLHVISQD FDSPCLKNKK HWNSFNTEYF LESQAVIEMV QEAGRVTVRD GMPELLKLPL RCHECQQLLP SIPQLKEHLR KHWTQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
