LXA4R, which was first identified based on sequence homology as the formyl peptide receptor like receptor (FPRL1). Lipoxin signaling through the LXA4R inhibits chemotaxis, transmigration, superoxide generation and NF-kappa B activation. Bao et al. (1992) identified 2 structural homologs of formyl peptide receptor (FPR1), FPRL1 (FPR2) and FPRL2 (FPR3). Since these 2 structural homologs did not recognize the ligand FMLP, Bao et al. (1992) referred to them as orphan receptors. Nonetheless, they probably function as chemotactic receptors. Prat et al. (2006) found that a Staphylococcus aureus protein, which they called FLIPr, bound to and impaired neutrophil responses to FPRL1 agonists in terms of calcium mobilization and directed migration. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
