PbpD, Recombinant, Bacillus Subtilis, aa213-450, His-SUMO-Tag (Penicillin-binding Protein 4)
Artikelnummer:
USB-370737
Hersteller Artikelnummer:
370737
Alternativnummer:
USB-370737-20,USB-370737-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Preferential cleavage: Ac2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine. Source: Partial recombinant protein corresponding to aa213-450 from Bacillus subtilis PbpD, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~43kD Amino Acid Sequence: PNNPTLYDPLKHFDYTKSRQERLLKGLKDAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDGVKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIEQTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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