DNA Ligase, Recombinant, Colwellia Psychrerythraea, aa330-689, His-tag (ligA)

Produktübersicht

• Artikelname: DNA Ligase, Recombinant, Colwellia Psychrerythraea, aa330-689, His-tag (ligA)
• Artikelnummer: USB-405910
• Hersteller Artikelnummer: 405910
• Alternativnummer: USB-405910-20,USB-405910-100,USB-405910-1
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

DNA ligase that catalyzes the formation of phosphodiester linkages between 5-phosphoryl and 3-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. Source: Recombinant protein corresponding to aa330-689 from Colwellia Psychrerythraea DNA Ligase, fused to His-tag, at N-terminal, expressed in E. coli. Molecular Weight: ~42.1kD AA Sequence: KFPAEEELTCVEDVEFQVGRTGAITPVARLKPVFVGGVTVSNATLHNQDEITRLGLKVNDFVVIRRAGDVIPQIVSVVLDKRPDNAVDIVFPTSCPVCDSAVAKPEGEAVLRCTAGLFCAAQRKEAIKHFASRKAHDVDGLGDKLVEQLVDEKLINTPADLFKLTEIQVSTIDRMGKKSATNLINGLEQAKSTTLAKFIYGLGIREVGEATAANLANHFYTLAAIESASLEDLQNVSDVGEVVAKNIINFFKEEHNLAIVSGLSEVMHWPTIEIKSAEELPLAEQIFVLTGTLTQMGRTEAKTALQSLGAKVSGSVSKNTHFVVAGDKAGSKLTKAQDLGISVLTEDGLVALLAEHGITI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Molekulargewicht: 42.1
• UniProt: Q47YI0
• Reinheit: ~85% (SDS-PAGE)
• Formulierung: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.