DNA Ligase, Recombinant, Colwellia Psychrerythraea, aa330-689, His-tag (ligA)

Product Overview

• Article Name: DNA Ligase, Recombinant, Colwellia Psychrerythraea, aa330-689, His-tag (ligA)
• Biozol Catalog Number: USB-405910
• Supplier Catalog Number: 405910
• Alternative Catalog Number: USB-405910-20,USB-405910-100,USB-405910-1
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

DNA ligase that catalyzes the formation of phosphodiester linkages between 5-phosphoryl and 3-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. Source: Recombinant protein corresponding to aa330-689 from Colwellia Psychrerythraea DNA Ligase, fused to His-tag, at N-terminal, expressed in E. coli. Molecular Weight: ~42.1kD AA Sequence: KFPAEEELTCVEDVEFQVGRTGAITPVARLKPVFVGGVTVSNATLHNQDEITRLGLKVNDFVVIRRAGDVIPQIVSVVLDKRPDNAVDIVFPTSCPVCDSAVAKPEGEAVLRCTAGLFCAAQRKEAIKHFASRKAHDVDGLGDKLVEQLVDEKLINTPADLFKLTEIQVSTIDRMGKKSATNLINGLEQAKSTTLAKFIYGLGIREVGEATAANLANHFYTLAAIESASLEDLQNVSDVGEVVAKNIINFFKEEHNLAIVSGLSEVMHWPTIEIKSAEELPLAEQIFVLTGTLTQMGRTEAKTALQSLGAKVSGSVSKNTHFVVAGDKAGSKLTKAQDLGISVLTEDGLVALLAEHGITI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 42.1
• UniProt: Q47YI0
• Purity: ~85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.