Epidermal Growth Factor Receptor, T790M, Recombinant, Human, GST-Tag (erbB, EGFR)

Produktübersicht

• Artikelname: Epidermal Growth Factor Receptor, T790M, Recombinant, Human, GST-Tag (erbB, EGFR)
• Artikelnummer: USB-E3375-47D
• Hersteller Artikelnummer: E3375-47D
• Alternativnummer: USB-E3375-47D-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Human EGFR T790M (GenBank Accession No. NM_005228), (aa668-1210) with N-terminal GST tag. Applications: Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Specific Activity: ~0.9 pmol/min/ug Recommended Dilution: Assay Conditions: 50mM HEPES, pH 7.5, 10mM of MgCl2, 1mM of EDTA, 0.01% Brij-35, 200uM ATP. Substrate 2uM Tyr peptide 4 at RT for 1 hour. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Molekulargewicht: 88
• NCBI: 005228
• UniProt: P00533
• Reinheit: ~40%
• Formulierung: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 138mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.