Protein Kinase A, Substrate Peptide (PKA)

Artikelnummer: USB-P9102-91D
Artikelname: Protein Kinase A, Substrate Peptide (PKA)
Artikelnummer: USB-P9102-91D
Hersteller Artikelnummer: P9102-91D
Alternativnummer: USB-P9102-91D-2
Hersteller: US Biological
Kategorie: Molekularbiologie
9 residue synthetic peptide [GRTGRRNSI-NH2], which is a specific substrate of PKA (Km~0.11uM). When compared to Kemptide, the Km of PKA Substrate Peptide is 40 fold lower and Vmax is two times higher. Applications: Kinase Assay: Tested using 250ng of PKA catalytic subunit to phosphorylate 10uM of the PKA Substrate Peptide. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O or buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht: 1016
NCBI: 002730
UniProt: P17612
Reinheit: 95% (HPLC)
Formulierung: Supplied as a lyophilized powder, reconstitute with sterile ddH2O or assay dilution buffer