Protein Kinase A, Substrate Peptide (PKA)

Product Overview

• Article Name: Protein Kinase A, Substrate Peptide (PKA)
• Biozol Catalog Number: USB-P9102-91D
• Supplier Catalog Number: P9102-91D
• Alternative Catalog Number: USB-P9102-91D-2
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

9 residue synthetic peptide [GRTGRRNSI-NH2], which is a specific substrate of PKA (Km~0.11uM). When compared to Kemptide, the Km of PKA Substrate Peptide is 40 fold lower and Vmax is two times higher. Applications: Kinase Assay: Tested using 250ng of PKA catalytic subunit to phosphorylate 10uM of the PKA Substrate Peptide. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O or buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 1016
• NCBI: 002730
• UniProt: P17612
• Purity: 95% (HPLC)
• Form: Supplied as a lyophilized powder, reconstitute with sterile ddH2O or assay dilution buffer