TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

Artikelnummer: USB-T2000
Artikelname: TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)
Artikelnummer: USB-T2000
Hersteller Artikelnummer: T2000
Alternativnummer: USB-T2000-10,USB-T2000-25,USB-T2000-50,USB-T2000-100
Hersteller: US Biological
Kategorie: Biochemikalien
TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Graces insect medium. Sucrose, fructose and several Krebs cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. Appearance: White to off white, homogenous, free flowing powder. Solubility: Colorless to light yellow, clear, complete. pH: 6.5 0.5