TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)

Product Overview

• Article Name: TC-100 Insect Medium w/ L-Glutamine, NaHCO3 (Powder)
• Biozol Catalog Number: USB-T2000
• Supplier Catalog Number: T2000
• Alternative Catalog Number: USB-T2000-10,USB-T2000-25,USB-T2000-50,USB-T2000-100
• Manufacturer: US Biological
• Category: Biochemikalien

Product Description

TC-100 is a modification done by Dr. Gardiner and Dr. Stockdale of the original formulation of Graces insect medium. Sucrose, fructose and several Krebs cycle intermediates were omitted from the original formula. The modification was done to optimize the production of Autographia californica NPV virions by cells from the fall armyworm, Spodoptera frugiperda. TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. Appearance: White to off white, homogenous, free flowing powder. Solubility: Colorless to light yellow, clear, complete. pH: 6.5 0.5