Tissue, Total Protein, Human Adult Normal, Brain, Cerebellum (left) HisTek(TM)

Artikelnummer: USB-T5595-6423
Artikelname: Tissue, Total Protein, Human Adult Normal, Brain, Cerebellum (left) HisTek(TM)
Artikelnummer: USB-T5595-6423
Hersteller Artikelnummer: T5595-6423
Alternativnummer: USB-T5595-6423-1
Hersteller: US Biological
Kategorie: Sonstiges
Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. Applications: Electrophoresis, Western Blotting and Immunoprecipitation Enzymatic Activity Protein-DNA Interaction Protein-Protein Interaction Tissue-Specific Expression Identification Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex. Preparation: Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70C. Quality Control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -70C. Aliquots are stable for at least 6 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Formulierung: Supplied as a frozen liquid in HEPES, pH7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40, a cocktail of protease inhibitors.