Tissue, Total Protein, Human Adult Normal, Brain, Cerebellum (left) HisTek(TM)
Biozol Catalog Number:
USB-T5595-6423
Supplier Catalog Number:
T5595-6423
Alternative Catalog Number:
USB-T5595-6423-1
Manufacturer:
US Biological
Category:
Sonstiges
Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis. Applications: Electrophoresis, Western Blotting and Immunoprecipitation Enzymatic Activity Protein-DNA Interaction Protein-Protein Interaction Tissue-Specific Expression Identification Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex. Preparation: Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70C. Quality Control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -70C. Aliquots are stable for at least 6 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a frozen liquid in HEPES, pH7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40, a cocktail of protease inhibitors.
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