Tubulin, beta 3 (Tubulin beta III, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4), IgG1, Clone: [2Q121 TU-20], Mouse, Monoclonal
Artikelnummer:
USB-T9155-10
Hersteller Artikelnummer:
T9155-10
Alternativnummer:
USB-T9155-10-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ELISA, IHC, IP, WB
Immunogen:
Synthetic peptide corresponding to amino acids 443-450, ESESQGPK, of human class 3 beta-tubulin conjugated to KLH. Epitope: C-terminus.
Class 3 beta tubulin (beta 3-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class 3 beta tubulin is associated with the resistances of microtubule- targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors. Applications: Suitable for use in Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunocytochemistry (Fixed): 1:100-1:500. Immunohistochemistry (FFPE): 1:20-1:100. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Western Blot: 1:1000. Optimal dilutions to be determined by the researcher. Positive Control: Brain tissue or neurons of any type. Negative Control: Non-neuronal tissue or Astrocytes. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.