Uracil DNA Glycosylase (UNG), Clone: [k1C12], Mouse, Monoclonal

Produktübersicht

• Artikelname: Uracil DNA Glycosylase (UNG), Clone: [k1C12], Mouse, Monoclonal
• Artikelnummer: USB-U1900-06A
• Hersteller Artikelnummer: U1900-06A
• Alternativnummer: USB-U1900-06A-100
• Hersteller: US Biological
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: Recombinant protein corresponding to aa1-313 from human UNG, expressed in E. coli.

Produktbeschreibung

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Western Blot: 1:1000-1:2000. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [k1C12]
• Reinheit: Purified by Protein G affinity chromatography.
• Formulierung: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.