SIGNR7 (dendritic cell-specific ICAM-3 grabbing nonintegrin related gene 7), also known as CD209g, belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens. SIGNR7 is one of several mouse proteins which are encoded by separate genes and exhibit structural and sequence homology to human DC-SIGN and DC-SIGNR. Mouse SIGNR7 is a type 2 membrane protein that consists of a 45aa cytoplasmic domain, a 22aa transmembrane segment, and a 188aa extracellular domain with a juxtamembrane neck region and a single carbohydrate recognition domain (CRD). Within the CRD, mouse SIGNR7 shares 44%-49% aa sequence identity with mouse SIGNR1, 2, 3, 4, and 5 and 81% with SIGNR8. It shares 44%-54% aa sequence identity with the CRD of bovine SIGNR7, human DC-SIGN, human DC-SIGNR, and rat DC-SIGN. A SIGNR7 ortholog has been described in cow but not in human. SIGNR7 binds both fucose and mannose residues and shows a distinct binding preference for the 6-sulfo-sialyl Lewisx carbohydrate. Its interaction with mannosylated BSA is disrupted at low pH. Source: Murine myeloma cell line, NS0 derived recombinant protein corresponding to Arg71-Tyr255 from mouse DC-SIGNR7, with an N-terminal 6-His tag SDS-PAGE: 29-30kD, reducing conditions. Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of LS180 human colon adenocarcinoma cells. When 5x10e4 cells/well are added to rmSIGN-R7 coated plates (5ug/ml,100ul/well), approximately 70%-90% will adhere after 1 hour at 37C. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
