Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Pri... The New England Journal of Medicine, 2020
Vaccines to prevent coronavirus disease 2019 (Covid-19) are urgently needed. The effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines on viral replication in both upper and lower airways is important to evaluate in nonhuman primates. In this study, nonhuman primates received 10 or 100 μg of mRNA-1273, a vaccine encoding the prefusion-stabilized spike protein of SARS-CoV-2, or no vaccine. Antibody and T-cell responses were assessed before upper- and lower-airway challenge with SARS-CoV-2. Active viral replication...
Plasma cell survival in the absence of B cellmemory Nature Communications, 2017
Abstract Pre-existing serum antibodies play an important role in vaccine-mediated protection againstinfection but the underlying mechanisms of immune memory are unclear. Clinical studiesindicate that antigen-specific antibody responses can be maintained for many years, leadingto theories that reactivation/differentiation of memory B cells into plasma cells is required tosustain long-term antibody production. Here, we present a decade-long study in which wedemonstrate site-specific survival of bone marrow-derived plasma cells and durable an...
Mechanical regulation of glycolysis via cytoskeleton architecture Nature, 2020
Recently, Park et al. identified an actin-TRIM21-PFK mechanistic pathway utilized by cells to adapt their metabolic activity in response to mechanical cues; this is the first report to identify a direct link between mechanical inputs from the cell environment and metabolic responses. Human bronchial epithelial cells (HBECs) were exposed to stiff or soft substrates to mimic environmental mechanical stimuli. Analysis of HBECs exposed to these different stimuli, using an array of metabolic, biological, and molecular tools, identified a signif...
Histopathology and ultrastructural findings of fatal COVID-19 infectio... The Lancet, 2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic, with increasing deaths worldwide. To date, documentation of the histopathological features in fatal cases of the disease caused by SARS-CoV-2 (COVID-19) has been scarce due to sparse autopsy performance and incomplete organ sampling. Bradley at al. aimed to provide a clinicopathological report of severe COVID-19 cases by documenting histopathological changes and evidence of SARS-CoV-2 tissue tropism. The primary pathology observed in this cohort...
Microglial Depletion with Clodronate Liposomes Increases Proinflammato... Mol Neurobiol., 2019
Abstract Investigators are increasingly interested in using microglial depletion to study the role of microglia under pathologic conditions. Liposome-encapsulated clodronate is commonly used to eliminate macrophage populations because it causes functionally irreversible inhibition and apoptosis once phagocytized by macrophages. Recent studies have shown that microglia can be depleted in disease models by injecting clodronate liposomes into the brain parenchyma. However, it is unclear whether intracerebral administration of...
Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infe... Cell Reports, 2020
A team of scientists, including researchers from Active Motif China, developed recombinant antibodies targeting SARS-CoV-2 and their work was recently published in the journal Cell Reports. Several of these antibodies were strong binders to the SARS-CoV-2 Spike S1 protein and were able to effectively neutralize infectivity of the virus, suggesting that they could be further developed and used as therapeutics against COVID-19. Learn in the following article how Wan et al. identified 11 potent neutralizingantibodies against COVID-19 from 11con...
Immunological and inflammatory profiles in mild and severe cases of CO... Nat Commun 11, 2020
Our partner AimPlex leverages years of assay development expertise to offer comprehensive solutions in biomedical testing for multiplexed bead-based immunoassays. Founded in August 2011, the company is committed to developing and providing high-quality, cost-effective assay reagents for flow cytometry analyses. AimPlex immunoassay products employ existing flow cytometry analytical platforms for high-sensitivity detection. In the following work, Song et al. were for the first time able to study the immunological and inflammatory profiles of COVID-19 pa...
Vitreous Levels of Lipocalin-2 on Patients With Primary Rhegmatogenous... PLoS One, 2019
Our partner AimPlex leverages years of assay development expertise to offer comprehensive solutions in biomedical testing for multiplexed bead-based immunoassays. Founded in August 2011, the company is committed to developing and providing high-quality, cost-effective assay reagents for flow cytometry analyses. AimPlex immunoassay products employ existing flow cytometry analytical platforms for high-sensitivity detection. In the following notheworthing study conducted by Georgios Batsos et al., Li...
Prostaglandin F2α influences pre-ovulatory follicle characteristics an... Theriogenology, 2020
Highlights Treatment of anovular cows with a second dose of 25 mg of prostaglandin F2α increased pregnancy per AI in normothermic cows. Treatment with prostaglandin F2α had minor impacts on LH pulsatility, but increased the size and estradiol content in the pre-ovulatory follicle. Prostaglandin has a role in fertility of anovular cows that is unrelated to its luteolytic effect. In a recent
Heightened Innate Immune Responses in theRespiratory Tract of COVID-19... Cell Host & Microbe
SUMMARY The outbreaks of 2019 novel coronavirus disease (COVID-19) caused by SARS-CoV-2 infection have posed asevere threat to global public health. It is unclear how the human immune system responds to this infection.Here, we used metatranscriptomic sequencing to profile immune signatures in the bronchoalveolar lavage fluidof eight COVID-19 cases. The expression of proinflammatory genes, especially chemokines, was markedlyelevated in COVID-19 cases compared to community-acquired pneumonia patients and healthy controls, sug-gesting that SARS...