Antibody-Drug Conjugates

ADC Conjugation Kit from ACROBiosystems

ACROBiosystems is a leading manufacturer of recombinant proteins and other critical reagents to support the development of target therapeutics. The company employs an application oriented development strategy, with a particular focus on product design, quality control and solution based support. Their products and services enable anyone in the field of drug development to have a more intuitive and streamlined process.

 

ADC Conjugation Kit — Conjugation with Speed, Precision, and Reliability

Antibody-Drug Conjugates (ADCs) are targeted therapeutics structured as "Antibody-Linker-Payload". By precisely conjugating cytotoxic payloads (e.g., MMAE, DXd) to antibodies, ADCs enable targeted delivery of toxins to tumor cells. Conjugation technology critically influences ADC efficacy, safety, and manufacturability.

ACROBiosystems has developed a new ADC Conjugation Kit, delivering a rapid, precise, and robust ADC preparation process. Generate functional ADCs within 3 hours while maintaining antibody activity and high recovery. The resulting conjugates are suitable for direct use in cellular or animal studies to accelerate candidate ADC screening.

 

Product Features

• Fast: Complete ADC prep in <3 hours
• Precise: Controlled DAR 4/8 with preserved antibody activity
• Efficient: >95% conjugation, >80% recovery
• Low Input: Compatible with ≥2 mg/mL antibodies
• Simple: 15 min purification via spin column

 

Technology & Principle

Based on interchain cysteine conjugation technology, this kit reduces antibody disulfide bonds to generate reactive cysteine residues for non-site-specific maleimide conjugation. This classic strategy enables rapid, controlled ADC preparation—requiring only 3h to obtain ADC conjugates with homogeneous DAR. The resulting conjugates demonstrate significant cytotoxicity, offering a robust tool for ADC screening and mechanistic studies.

Protocol Overview

1. Prepare antibody solution for 2 mg/mL in 1×PBS buffer (pH7.2-7.4)
2. Antibody reduction 37°C, 1h
3. Antibody conjugation 23°C, 1h
4. Reaction quenching RT, 0.5h
5. Purification and buffer change

 

Product List

Art. N°	Molecule	Product Description
ABS-ADC-P013	MMAE (Monomethyl Auristatin E)	ADC Conjugation Kit (MMAE, DAR4, 200ug, for human IgG1)
ABS-ADC-P014	MMAE (Monomethyl Auristatin E)	ADC Conjugation Kit (MMAE, DAR4, 1mg, for human IgG1)
ABS-ADC-P015	MMAF (Monomethyl Auristatin F)	ADC Conjugation Kit (MMAF, DAR4, 200ug, for human IgG1)
ABS-ADC-P016	MMAF (Monomethyl Auristatin F)	ADC Conjugation Kit (MMAF, DAR4, 1mg, for human IgG1)
ABS-ADC-P017	Exatecan	ADC Conjugation Kit (Exatecan, DAR8, 200ug, for human IgG1)
ABS-ADC-P018	Exatecan	ADC Conjugation Kit (Exatecan, DAR8, 1mg, for human IgG1)
ABS-ADC-P021	Exatecan	ADC Conjugation Kit (Exatecan, 200ug, for human IgG4&mouse IgG)
ABS-ADC-P022	Exatecan	ADC Conjugation Kit (Exatecan, 1mg, for human IgG4&mouse IgG)