Protein Analysis

Empowering your Workflow

    

Workflow: Protein Analysis

From Sample to Insight. Empowering your Workflows.

Protein analysis encompasses a broad set of techniques used to study the structure, function, and interactions of proteins within biological systems. It is essential for understanding cellular mechanisms, disease pathways, and biomolecular behavior.

This workflow supports applications ranging from basic research to drug discovery and biomarker development.

 

---

Step 1: Sample Preparation

 

Your Experimental Methods

• Cell / Tissue lysis: Mechanical Lysis, Chemical Lysis (Detergent-based), Enzymatic Lysis, Osmotic Lysis, Sonication, Freeze-Thaw Lysis, Bead Beating, Homogenization
• Protein Extraction: Total Protein Extraction, Cytoplasmic Protein Extraction, Nuclear Protein Extraction, Membrane Protein Extraction, Mitochondrial Protein Extraction, Secreted Protein Isolation, Organelle-specific Extraction
• Supernatant Collection: Centrifugation, Differential Centrifugation, Ultracentrifugation, Filtration, Clarification, Debris Removal

 

Our Supporting Brands

 

Protocols and Method Papers from our Suppliers

• Protein Isolation: How to Choose Membrane Protein Isolation Kits with Invent Biotechnologies

 

Product Highlights

	Cytoplasmic and Nuclear Protein Extraction Kit Complete set of extraction reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and fresh tissues. Manufacturer: Boster Bio Product number: BOB-AR0106
	Mobicol Spin Ni-IDA (His-tagged proteins) Ni-IDA columns for easy, fast, and cost-effective routine purification of His-tagged proteins under native and denaturing conditions. Manufacturer: BIOZOL Product number: MOB-PR-HTK110

 

Featured Topics

• Mobicol Mini Columns: Incredibly versatile chromatography columns
• Extracellular Vesicles (EV): Standards, kits and reagents for your research
• SpinMate Ultrafiltration Concentrators from Protein Ark: Greater efficiency in protein purification
• Saliva Samples: Sample collection and specialized assays
• Anatrace Lipids: Fos-Choline® detergents

 

---

Step 2: Protein Processing

 

Your Experimental Methods

• Denaturation: Heat Denaturation, Chemical Denaturation (Urea, Guanidine HCl), Detergent-based Denaturation (SDS), Reducing Conditions (DTT, β-Mercaptoethanol, TCEP), Non-reducing Sample Preparation
• Digestion: In-solution Digestion, In-gel Digestion, Enzymatic Digestion (Trypsin, Lys-C, Chymotrypsin, Glu-C, Asp-N, Proteinase K), Limited Proteolysis
• Modification: Protein Labeling, Biotinylation, Fluorescent Labeling, Crosslinking, PEGylation, Reduction & Alkylation, Deglycosylation, Phosphorylation / Dephosphorylation, Conjugation (z. B. Dye- oder Enzyme-Kopplung)

 

Our Supporting Brands

 

Product Highlight

MobiTEVProtease1 (HighSpeed), recombinant, His-Tag MobiTEV Protease1 (HighSpeed) is used to remove fusion tags and to release the „native“ protein of interest. Manufacturer: BIOZOL Product number: MOB-PR-ETA10010

 

Featured Topics

• Recombinant active enzymes: Biologically-active human recombinant proteins from SignalChem
• Bioconjugation: Vector SoluLINK available at BIOZOL

 

---

Step 3: Protein Detection & Separation

 

Your Experimental Methods

• Immunoassays: ELISA, CLIA, ECLIA, RIA, Lateral Flow Assays (LFIA), Multiplex Immunoassays, AlphaLISA/AlphaScreen, TR-FRET, Homogeneous Immunoassays
• Electrophoresis: SDS-PAGE, Native PAGE, Blue Native PAGE, Isoelectric Focusing (IEF), 2D-Gelelektrophorese (2D-PAGE), Capillary Electrophoresis
• Staining: Coomassie Staining, Silver Staining, Fluorescent Protein Stains, Zinc Staining, Negative Staining, Total Protein Staining, In-gel Activity Staining
• Blotting: Western Blot, Dot Blot, Slot Blot, Far-Western Blot, Southwestern Blot, Eastern Blot, Protein Transfer (Wet, Semi-dry, Dry Blotting)
• Antibody binding: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (Intracellular Staining), Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), Chromatin Immunoprecipitation (ChIP), CUT&RUN, CUT&Tag, ELISPOT
• Mass Spectrometry: MALDI-TOF, LC-MS/MS, Tandem MS (MS/MS), Top-down Proteomics, Bottom-up Proteomics, Targeted Proteomics (MRM/SRM, PRM), Quantitative Proteomics (TMT, iTRAQ, SILAC, Label-free), Native MS
• Activity Assays: Enzyme Activity Assays, Kinase Assays, Phosphatase Assays, Protease Assays, ATPase/GTPase Assays, Reporter Gene Assays, Cell-based Functional Assays, Fluorometric, Colorimetric und Luminescent Assays
• Crystallization: Protein Crystallization Screening, Optimization, Vapor Diffusion (Hanging Drop, Sitting Drop), Microbatch, Lipidic Cubic Phase (LCP), Serial Crystallography, Cryoprotection
• Interaction Studies: Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), Isothermal Titration Calorimetry (ITC), Microscale Thermophoresis (MST), Förster Resonance Energy Transfer (FRET), Bioluminescence Resonance Energy Transfer (BRET), Yeast Two-Hybrid (Y2H), Pull-down Assays, Co-IP, Crosslinking Studies, Protein Microarrays, Thermal Shift Assays (DSF), Biophysical Binding Assays

 

Our Supporting Brands

 

Protocols and Method Papers from our Suppliers

• ELISA: Triplicates, Duplicates, or Single Measurements
• Flow Cytometry: Calibration and Performance Tracking
• Immunohistochemistry (IHC): Atlas Antibodies Standard IHC Protocol 
• Western Blot: Principle and Method with MBL
• Western Blot: Troubleshooting Tips from GeneTex
• Western Blot: Blocking with immunizing peptide
• Western Blot: Fluorescent Western Blotting

 

Product Highlights

	Control Beads Blocked agarose beads for use as a negative control with Echelon’s Lipid Coated Beads for protein pulldown experiments. Manufacturer: Echelon Biosciences Product number: ECH-P-B000
	HISTOPRIME Normal Serum Blocking sera for immunohistochemistry, manufactured in Germany. Many species available from stock and more on request. Manufacturer: BIOZOL Product number: various
	Anti-His Epitope Tag (rec) from Mouse [13/45/31] Recombinant monoclonal antibody that specifically detects any kind of histidine-tagged proteins in cells and complex cellular lysates. Manufacturer: BIOZOL Product number: DNA-DIA-900-100
	GloMeltTM Thermal Shift Protein Stability Kit Detection of protein unfolding or protein thermal stability by performing a thermal shift assay, also called differential scanning fluorimetry. Manufacturer: Biotium Product number: BOT-33021
	Morpheus Complete HT-96 kit Morpheus is a 96 condition 3D protein crystallisation screen incorporating a range of low molecular-weight ligands. Morpheus I, II and III included. Manufacturer: Molecular Dimensions Product number: MDL-MD1-124

 

Featured Topics

• Host Cell Proteins (HCPs): Efficient detection methods
• The Human Protein Atlas: All the human proteins mapped in cells, tissues and organs
• Atlas Antibodies MolBoolean™: Unlock the secrets of protein-protein interactions
• ChIP-Exo-Seq: Validated Antibodies from Atlas Antibodies
• 96 Well Plates: Coated microplates for immunoassays
• Collagen Detection: ELISA Kits for type I and type II collagen quantification
• Cell Adhesion Molecules (CAMs): Key Targets Covered by Biorbyt
• Multiplex IHC: AtlasPlex kits from Atlas Antibodies
• Complement System: WIESLAB® Functional Assays from Svar
• Anti-Phospho Antibodies from Affinity Biosciences: Highly specific detection of phosphorylated proteins
• Type I and Type II Interferons: High-purity proteins, sensitive ELISA Kits
• Collagen Hybridizing Peptide: Direct detection of unfolded collagen
• QuickSwitch™ Peptide Screening Kit from MBL
• Flow Cytometry: Research and Diagnostics
• ELISpot and FluoroSpot: Quantification of immune responses at the single-cell level
• Uncoated ELISA Kits from Elabscience: The cost-saving choice

 

---

 

Step 4: Visualization

 

Your Experimental Methods

• Secondary Reagents-based Signal Amplification: Secondary Antibodies, Polymer Detection Systems, Streptavidin-Biotin Systems, Tyramide Signal Amplification (TSA), Avidin-Biotin Complex (ABC), PAP/APAP Methods
• Chemiluminescent Detection: ECL, Enhanced Chemiluminescence, HRP Substrates, AP Chemiluminescent Substrates
• Fluorescent Detection: Fluorescent Secondary Antibodies, Near-Infrared (NIR) Detection, Multiplex Fluorescence
• Colorimetric Detection: Chromogenic HRP Substrates (DAB, TMB), AP Substrates (BCIP/NBT, Fast Red)
• Signal Enhancement & Mounting: Antifade Mounting Media, Nuclear Counterstains (DAPI, Hoechst), Signal Enhancers

 

Our Supporting Brands

 

Product Highlights

	Protein Detective Protein Detective is a one-step ultrafast Coomassie protein gel stain that requires no pre or post gel treatment. Manufacturer: BIOZOL Product number: BZL-PD1
	Western Blot Detection Trial Kit (Internal Controls) Includes anti-GAPDH [GT239], anti-β-Actin [HL1926], Trident femto western HRP substrate solutions and Trident prestained protein ladder. Manufacturer: GeneTex Product number: GTX444069

 

Featured Topics:

• Protein Detective: Your sidekick for protein staining
• Fluorescent Optotracers: Visualize protein aggregates, carbohydrate structures, and bacterial ECM
• Amytracker fluorescent tracers: High-quality visualization of amyloids
• Vector Labs Mounting Media: VECTASHIELD for IF and VectaMount for IHC
• AbBy Fluor® Dyes: Antibody conjugates from Bioss
• Fab Fragment Secondary Antibodies: Blocking of endogenous immunoglobulins
• Secondary Antibodies: Alexa Fluor® 555 and Alexa Fluor® 568 conjugates from Jackson ImmunoReasearch

 

back to top