CAR-T Therapy
Assays for Basic Research and Development - now 20% off!
Elabscience specializes
in immunodiagnostic technology for the Life Science community. Major products are antibodies, ELISA kits, proteins, labeling Kits, CLIA kits and related reagents.
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FINAL SALE 2026: 20% off all products from Elabscience. Quote: BIOZOL-FINAL2026 Valid until: December 15, 2026 |
CAR-T Turning T Cells into Tumor Hunters
CAR-T (Chimeric Antigen Receptor T cell) therapy is a breakthrough in cellular immunotherapy. Engineered T cells recognize tumor-specific antigens and selectively target malignant cells through precise antigen recognition and T-cell activation.
Upon antigen engagement, CAR-T cells release cytotoxic molecules such as Perforin and Granzyme B to eliminate tumor cells. They also secrete cytokines that activate other immune cells, while persistent memory-like populations support long-term immune surveillance and tumor control.
CAR-T therapy has transformed the treatment of hematological malignancies and holds promise for solid tumors. Elabscience® offers integrated solutions supporting CAR-T research from basic investigation to preclinical development.
In Vitro Functional Characterization of CAR-T Cells
In vitro cytotoxicity assessment of CAR-T cells
The in vitro cytotoxic activity of CAR-T cells represents the most direct evidence of their therapeutic potential. At its core, this process reflects the engineered T cells’ precise recognition and efficient elimination of target cells. Mechanistically, target cell killing is primarily mediated through two pathways: the granzyme/perforin-dependent cytotoxic pathway and the death receptor–mediated signaling pathway.
T cell cytotoxicity and toxicity assessment related products:
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Catalog No. |
Product Description |
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FITC Anti-Human Granzyme B Antibody [QA18A28] |
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PE Anti-Human Granzyme B Antibody [QA18A28] |
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Human GzmB (Granzyme B) ELISA Kit |
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Human PRF1 (Perforin 1) ELISA Kit |
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Mini Sample Human GzmB (Granzyme B) ELISA Kit |
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Uncoated Human GzmB (Granzyme B) ELISA Kit |
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Calcein AM/PI Double Staining Kit |
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Enhanced Cell Counting Kit 8 (WST-8/CCK8) |
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E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) |
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Lactate Dehydrogenase (LDH) Activity Assay Kit (WST-8 method) |
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Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit |
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MTT Cell Proliferation and Cytotoxicity Assay Kit |
Cytokine release assay detection
During long-term culture of CAR-T cells, the cytokine secretion profile serves as a critical indicator for evaluating cellular activity, functional status, and overall performance. The levels of these secreted factors are closely associated with CAR-T cell proliferation, cytotoxic potency, persistence, and potential toxicity.
Cytokine ELISA assay detection
CelaQuant™ ELISA kits are immunoassay products independently developed by Elabscience® for the precise quantitative detection of target proteins in cell-based samples. Focusing on the field of cellular analysis, Elabscience® addresses the rapidly growing demand for high-performance detection solutions. Through extensive validation across diverse cell models, these kits deliver accurate, stable, and reliable data support for cellular experiments. Built-in quality controls are included, and select targets incorporate core calibrators traceable to international standards, ensuring data accuracy and global comparability. This makes them an ideal choice for cell-based analytical applications.
Cytokine ELISA assay related product list:
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Catalog No. |
Product Description |
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CellaQuant™ Human IFN-γ (Interferon Gamma) ELISA Kit |
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CellaQuant™ Human IL-1α (Interleukin 1 Alpha) ELISA Kit |
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CellaQuant™ Human IL-1β (Interleukin 1 Beta) ELISA Kit |
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CellaQuant™ Human IL-2 (Interleukin 2) ELISA Kit |
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CellaQuant™ Human IL-6 (Interleukin 6) ELISA Kit |
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CellaQuant™ Human IL-8 (Interleukin 8) ELISA Kit |
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CellaQuant™ Human IL-10 (Interleukin 10) ELISA Kit |
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CellaQuant™ Human IL-15 (Interleukin 15) ELISA Kit |
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CellaQuant™ Human IL-17A (Interleukin 17A) ELISA Kit |
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CellaQuant™ Human IL-18 (Interleukin 18) ELISA Kit |
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CellaQuant™ Human IL-23 (Interleukin 23) ELISA Kit |
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CellaQuant™ Human IL-33 (Interleukin 33) ELISA Kit |
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CellaQuant™ Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit |
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CellaQuant™ Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit |
Cytokine ELISPOT assay detection
ELISPOT (enzyme-linked immunospot assay) is a highly sensitive technique capable of detecting and quantifying cytokine secretion by T cells (e.g., IFN-γ, IL-2, IL-4, IL-17) at the single-cell level. Each spot generated in an ELISPOT assay represents one cytokine-secreting T cell, enabling absolute quantification of the magnitude of the immune response and accurately reflecting the number of antigen-responsive T cells within a population. This feature makes ELISPOT a powerful tool for precisely assessing immune response strength and comparing immune status across different groups or time points. It not only confirms the presence of T cells but also directly and sensitively measures their functional immune responses.
Cytokine ELISPOT assay related product list:
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Catalog No. |
Product Description |
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Human IFN-γ (Interferon Gamma) ELISPOT Kit |
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Human IL-1β (Interleukin 1β) ELISPOT Kit |
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Human IL-2 (Interleukin 2) ELISPOT Kit |
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Human IL-4 (Interleukin 4) ELISPOT Kit |
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Human IL-5 (Interleukin 5) ELISPOT Kit |
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Human IL-6 (Interleukin 6) ELISPOT Kit |
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Human IL-10 (Interleukin 10) ELISPOT Kit |
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Human IL-17A (Interleukin 17A) ELISPOT Kit |
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Human TNF-α (Tumor Necrosis Factor Alpha) ELISPOT Kit |
Cell metabolic function assay detection
Metabolic profiling is a core technique for deciphering CAR-T cell fate, function, and efficacy. By systematically monitoring glycolysis, oxidative phosphorylation, amino acid and lipid metabolism, and mitochondrial function, it precisely links metabolic reprogramming to activation, proliferation, differentiation, stemness maintenance, and exhaustion.
In preclinical settings, metabolic assays guide optimization of culture conditions, co-stimulatory domains, and genetic modifications, thereby improving expansion efficiency, memory phenotype, and resistance to exhaustion. Clinically, metabolic parameters serve as predictive biomarkers for in vivo persistence, infiltration, and antitumor activity, supporting product quality assessment and dosing strategies.
Overall, metabolic testing is not only a mechanistic tool but also essential for CAR-T standardization, quality control, and clinical translation, offering significant value for developing next-generation CAR-T cells with enhanced persistence and functionality.
Cell metabolic assay related product list:
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Catalog No. |
Product Description |
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2-NBDG Glucose Uptake Cell-Based Kit |
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Alanine Aminotransferase (ALT/GPT) Activity Colorimetric Assay Kit |
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Aspartate Aminotransferase (AST/GOT) Activity Colorimetric Assay Kit |
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Arginase Activity Colorimetric Assay Kit |
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β-Hydroxybutyrate (Ketone Body) Colorimetric Assay Kit |
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Cholesterol Uptake Fluorometric Assay Kit |
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Cysteine (Cys) Colorimetric Assay Kit |
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Enhanced ATP Chemiluminescence Assay Kit |
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Enhanced Oxygen Consumption Rate (OCR) Fluorometric Assay Kit |
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Extracellular Acidification Rate (ECAR) Fluorometric Assay Kit |
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Fatty Acid Uptake Fluorometric Assay Kit |
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Fatty Acid Oxidation (FAO) Colorimetric Assay Kit |
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Glucose (GLU) Fluorometric Assay Kit |
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Glutamine (Gln) Colorimetric Assay Kit |
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Glutaminase (GLS) Activity Assay Kit |
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Glycolysis Stress Fluorometric Assay Kit |
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Homocysteine (Hcy) Colorimetric Assay Kit |
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L-Arginine (L-Arg) Colorimetric Assay Kit |
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Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit |
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L-Lactic Acid (LA) Colorimetric Assay Kit |
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Mitochondrial Membrane Potential Assay Kit (with JC-1) |
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Mitochondrial Stress Fluorometric Assay Kit |
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MitoBright Green Probe Assay Kit |
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MitoBright Red Probe Assay Kit |
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MitoBright Deep Red Probe Assay Kit |
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NADP+/NADPH Colorimetric Assay Kit (WST-8) |
22.10.2026