Multiplex IHC

AtlasPlex kits from Atlas Antibodies

Atlas Antibodies is the original manufacturer of highly validated primary antibodies and control antigens. The company was founded by researchers from the prestigious Human Protein Atlas project who wanted to make the unique antibodies developed in the project available to fellow researchers worldwide.

 

AtlasPlex: Multiplex IHC Made Simple

With AtlasPlex, you can build custom panels using a vast catalog of primary antibodies validated for IHC in the Human Protein Atlas. Choose with confidence from over 12,000 of high-quality, well-characterized antibodies, ensuring reliable performance and meaningful results in your research. No Secondary Antibodies needed.

 

Special Offer: Until September, we grant you 20% off all AltasPlex kits from Atlas Antibodies.

 

AtlasPlex from Atlas Antibodies is a flexible, ready-to-use multiplexing immunohistochemistry assay that brings high-throughput, iterative mIHC to FFPE tissue with unmatched clarity.

Designed to detect up to five biomarkers per day, AtlasPlex combines a complete, pre-validated TSA kit with 3 or 5 pre-biotinylated antibodies and tyramide dyes, eliminating the need for secondary antibodies and complex optimization. 

Choose from an unparalleled selection of more than 12,000 Human Protein Atlas–validated targets, you can design fully customizable panels without pre-set restrictions, ensuring maximum flexibility across diverse research areas. By expanding marker capacity, streamlining workflows, and delivering robust, reproducible signals, AtlasPlex allows you to focus on discovery rather than technical hurdles.

 

Visualize More with Flexible Multiplex IHC Panels

 

1) Build your own kit choosing from over 12,000 targets

With AtlasPlex, you have the flexibility to build your own custom multiplex IHC kit by selecting from over 12,000 validated targets. All antibodies are rigorously IHC-validated and supported by comprehensive data, including extensive validation images of tissue and organs from the Human Protein Atlas, ensuring high confidence in target detection and reliable, reproducible results for your research.

• Choose among ~12,000 targets
• No primary antibody host species restrictions
• Up to 5-plex/day for fast, flexible experimental design
• Bright, permanent tyramide signal, no fading overtime
• Low background, high specificity
• Fully compatible with standard fixed (FFPE) tissue workflows
• Ideal for spatial biology, tumor microenvironment, and biomarker discovery

 

2) Workflow: from sample to results in one day

AtlasPlex enables iterative multiplexed immunohistochemistry (mIHC) for 3-5 proteins detection in FFPE tissue.

1. Smart Complexing: Biotinylated primary antibodies (Ab1) are pre-bound to HRP, setting the stage for powerful signal amplification.
2. Targeted Tissue Binding: Apply the Ab1-HRP complex directly to fixed tissue. Binding is precise, reliable, and reproducible. 
3. Amplification: Tyramide-based signal amplification deposits a bright, permanent fluorescent signal at each target site, ensuring crisp, high-contrast images. 
4. Enzyme Quenching: Deactivate HRP quickly and cleanly. This rapid, gentle step completely replaces the need for harsh, tissue-damaging antibody stripping, ensuring no interference in downstream rounds. 
5. Repeat: Run multiple antibody cycles (Ab2...Ab5) with only 1.5 hours per cycle. Up to 5-plex per day. 
6. Brilliant Imaging: Capture your results using standard fluorescence microscopy. Reveal distinct biomarker landscapes with unmatched clarity.

 

3) Clear spectral coverage for enhanced visualization

Multichannel Fluorescent Imaging using Standard Wide-Field Microscope   AtlasPlex (3 Plex) included in the Kit: Tyramide 488 Tyramide 555 Tyramide 647   AtlasPlex (5 Plex) included in the Kit: Tyramide 488 Tyramide 555 Tyramide 647 Tyramide CF430 Tyramide 594

 

4) Less Time. Enhanced Sensitivity. Unlock Flexibility with AtlasPlex 

 

• Simplify Setup & Save Weeks - AtlasPlex delivers everything you need in one box, with pre-biotinylated antibodies and optimized TSA reagents, dramatically reducing setup and optimization time.
• Superior Sensitivity for Low-Abundance Proteins - AtlasPlex utilizes powerful TSA amplification with an integrated quencher to maximize signal-to-noise ratio.
• Unprecedented HPA Flexibility - AtlasPlex offers unparalleled flexibility, allowing you to incorporate a wide array of IHC-validated antibodies from the Human Protein Atlas, covering most human genes.
• Results in a Day Confident 5-Plex Staining - AtlasPlex is optimized for robust multiplexing, enabling clear visualization of up to 5 targets simultaneously in a single day.

 

Available AtlasPlex Kits

 

There are 4 AtlasPlex kit combinations to choose from: Standard (perfect for pilot studies or initial system testing) or Large (ideal for high-throughput or large-scale studies), 3 or 5 plex.

• Art. N° ATA-AP-S3 (Standard - 3 plex) → Includes reagents for approx. 25 reactions 
• Art. N° ATA-AP-L3 (Large - 3 plex) → Includes reagents for approx. 50 reactions 
• Art. N° ATA-AP-S5  (Standard - 5 plex) → Includes reagents for approx. 25 reactions 
• Art. N° ATA-AP-L5 (Large - 5 plex) → Includes reagents for approx. 50 reactions 

Each AtlasPlex Kit Includes:

• Biotinylated Antibodies  (Up to 5 customizable) 
• HRP Labeling & Quenching Buffers (Core components. No selection needed)
• Tyramide Dyes (Core components. no selection needed)