Workflow: Protein Analysis

From Sample to Insight. Empowering your Workflows.

Protein analysis encompasses a broad set of techniques used to study the structure, function, and interactions of proteins within biological systems. It is essential for understanding cellular mechanisms, disease pathways, and biomolecular behavior.

This workflow supports applications ranging from basic research to drug discovery and biomarker development.

Step 1: Sample Preparation

Your Experimental Methods

Cell / Tissue lysis: Mechanical Lysis, Chemical Lysis (Detergent-based), Enzymatic Lysis, Osmotic Lysis, Sonication, Freeze-Thaw Lysis, Bead Beating, Homogenization
Protein Extraction: Total Protein Extraction, Cytoplasmic Protein Extraction, Nuclear Protein Extraction, Membrane Protein Extraction, Mitochondrial Protein Extraction, Secreted Protein Isolation, Organelle-specific Extraction
Supernatant Collection: Centrifugation, Differential Centrifugation, Ultracentrifugation, Filtration, Clarification, Debris Removal

Our Supporting Brands

Protocols and Method Papers from our Suppliers

Protein Isolation: How to Choose Membrane Protein Isolation Kits with Invent Biotechnologies

Product Highlights

Cytoplasmic and Nuclear Protein Extraction Kit

Complete set of extraction reagents that enable the separation of nuclear protein and cytoplasmic fractions from cultured cells and fresh tissues.

Manufacturer: Boster Bio
Product number: BOB-AR0106

Mobicol Spin Ni-IDA (His-tagged proteins)

Ni-IDA columns for easy, fast, and cost-effective routine purification of
His-tagged proteins under native and denaturing conditions.

Manufacturer: BIOZOL
Product number: MOB-PR-HTK110

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