Rabbit IgG anti-Llama IgG (VHH)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Llama IgG (VHH)-unconj., MinX none
• Biozol Catalog Number: ROC-200-401-GM6S
• Supplier Catalog Number: 200-401-GM6S
• Alternative Catalog Number: ROC-200-401-GM6S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,WB
• Species Reactivity: Llama
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-VHH Antibody, single-domain antibody (sdAb), camelid VHH antibody
• Format: IgG
• Target Specificity: IgG (VHH)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.3 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Llama
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Application Notes: Anti-VhH antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Some cross-reactivity to E.coli proteins may be observed.

Images

Western Blot of Rabbit anti-VHH antibody. Lane 1: Ladder (Opal Prestained). Lane 2: VHH protein (p/n 000-001-GM6). Lane 3: Llama IgG1 protein (p/n 025-0140). Lane 4: Llama IgG2 protein (p/n 025-0144). Lane 5: Llama IgG3 protein (p/n 025-0143). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4C. Secondary antibody: Gt-a-Rb HRP (611-103-122) rabbit secondary antibody at 1:70,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit anti-VHH antibody. Lane 1: MW ladder (opal pre-stained) p/n (MB-210-0500). Lane 2: Llama IgG1 protein (p/n 025-0140). Lane 3: Llama IgG2 protein (p/n 025-0144). Lane 4: Llama IgG3 protein (p/n 025-0143). Lane 5: VhH protein (p/n 000-001-GM6). Load: 50 ng per lane. Primary antibody: VHH antibody at 1:1000 for overnight at 4C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:40,000 for 45 min at RT. Block: BlockOut (p/n MB-070) overnight at 4C. Predicted/Observed size: expect 15 and 18 kda band in VHH protein and reactivity with Llama IgG isotypes.

Western Blot of Rabbit Anti-VHH Antibody detecting VHH. Lane 1: MW Ladder Opal pre-stained (p/n MB-210-0500). Lane 2: VHH. Load: 0.50 µg per lane. Primary Antibody: Anti-VhH Antibody (p/n 200-401-GM6) at 1 ug/mL for overnight at 4°C. Secondary Antibody: Rabbit Secondary HRP Antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: MB-070 at RT for 30 min. Predicted/Observed size: ~15 kDa.

Western Blot of Rabbit anti-VHH Antibody. Lane 1: Hela whole cell lysate spiked with VHH single-domain antibody. Load: 10 µg lysate with 25 ng VHH. Primary antibody: Rabbit anti-VHH Antibody at 1:1,000 o/n at 4C. Secondary antibody: HRP Gt-a-Rb IgG (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for VHH.