Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by researcher.
ELISA results using Goat Anti-Human IgG. Influence of index case onM. lepraeinfection levels. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibody levels in children and adolescents were measured by ELISA, using either protein A, anti- IgM or anti-IgG to detect responses. Ina, samples were stratified by reported WHO operational classification of the index case as either MB (n=66) or PB (n=16). Inb, samples were stratified by estimated duration of exposure to the index leprosy case as either less than 10years (n=45) or greater than 10years (n=37). Data are displayed as box and whisker plots, with the box representing the Q1 to Q3 interquartile range and the horizontal bar representing the median of the optical density of the samples. Individual dots indicate outliers, andp-values are indicated by the lines above each indicated group. Fig. 3. PMID: 31196008.
ELISA results using Goat Anti-Human IgG.Impact of behavioral variables onM. lepraeinfection levels. Anti-natural octyl disaccharide-leprosy IDRI diagnostic (NDO-LID) antibody levels in children and adolescents were measured by ELISA, using either protein A, anti- IgM or anti-IgG to detect responses. Ina, samples were stratified by recorded knowledge of eating armadillo meat as either yes (n=14) or no (n=64). Inb, samples were stratified by recorded knowledge of BCG re-vaccination following identification of the index leprosy case as either yes (n=54) or no (n=16). Data are displayed as box and whisker plots, with the box representing the Q1 to Q3 interquartile range and the horizontal bar representing the median of the optical density of the samples. Individual dots indicate outliers, andp-values are indicated by the lines above each indicated group. Fig. 2. PMID: 31196008.
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