Mouse IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: Mouse IgG (H&L) Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-210-4302
• Supplier Catalog Number: 210-4302
• Alternative Catalog Number: ROC-210-4302
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Mouse IgG whole molecule
• Conjugation: HRP
• Alternative Names: Rabbit anti-Mouse IgG Peroxidase Conjugated Antibody, Rb anti-Mouse IgG HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: This product is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Images

Whole-cell serum IgG ELISA titres of mice immunized with alum, TbpA or Hp7-L10 when captured by(A)heat-killedN. gonorrhoeaestrain MS11 or(B)heat-killedN. meningitidisstrain M982. Female mice were used in the lower genital tract gonococcal colonization, while male mice were challenged systemically withN. meningitidis. Significance was determined as a significant increase in titer compared with mice that received alum alone by ordinary one-way ANOVA withpost-hocanalysis by Dunnetts multiple comparisons test. ****p< 0.0001. Figure 6. PMID: 30837995.

In vitrocharacterization of the LCL scaffold and the LCL-TbpA hybrid antigens.(A)Mouse sera were evaluated in a whole cell ELISA against both wild-typeN. meningitidisM982 (Black) and M982 with TbpB knocked out (M982deltatbpB, Gray). Sera were assayed in triplicate from individual mice (4 to 5 mice per group) and averaged and displayed as mean +/- SEM. Significance was determined as a significant increase in titer compared with mice that received adjuvant alone by two-way ANOVA with Sidaks multiple comparison test for both wildtype and knockout data sets. **p 0.01, ****p 0.0001. Figure 5. PMID: 30837995.