Rabbit IgG anti-Mouse IgM (H+L)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Mouse IgM (H+L)-HRPO, MinX none
• Biozol Catalog Number: ROC-210-4307
• Supplier Catalog Number: 210-4307
• Alternative Catalog Number: ROC-210-4307
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Mouse
• Immunogen: Anti-Mouse IgM (H&L) was produced by repeated immunization with mouse IgM whole molecule in rabbit.
• Conjugation: HRPO
• Alternative Names: Mouse IgM (H&L) Antibody, Rb-a-Mouse IgM (H&L) Peroxidase conjugated, Mouse IgM (H&L) Peroxidase Antibody in rabbit, Rabbit peroxidase conjugated Secondary Antibody, rabbit anti-Mouse IgM HRP Conjugated Antibody
• Format: IgG
• Target Specificity: IgM (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 10 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mouse
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application Notes: This secondary peroxidase conjugated antibody anti-Mouse has been tested by dot blot and ELISA and is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Mouse IgM (H&L) perox

Images

Dot Blot results of Rabbit Anti-Mouse IgM Antibody Peroxidase Conjugated. Dots are Mouse IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 30 min at RT. Primary Antibody: none. Secondary Antibody: Rabbit anti-Mouse IgM-HRP at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, Chemi filter.