Rabbit IgG anti-Swine IgG (H+L)-unconj., MinX none

Catalog Number: ROC-214-4102
Article Name: Rabbit IgG anti-Swine IgG (H+L)-unconj., MinX none
Biozol Catalog Number: ROC-214-4102
Supplier Catalog Number: 214-4102
Alternative Catalog Number: ROC-214-4102
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Porcine
Immunogen: Swine IgG whole molecule
Conjugation: Unconjugated
Alternative Names: rabbit anti-Swine IgG Antibody, rabbit anti Swine IgG
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Clonality: Polyclonal
Concentration: 10.0 mg/ml by UV absorbance at 280 nm
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Swine
Antibody Type: Secondary Antibody
Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Application Notes: Anti-Swine antibody is tested by Western blot and suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Western Blot of Rabbit anti-Swine IgG Antibody. Lane 1: Swine IgG (p/n 014-0102). Load: 100 ng per lane. Primary antibody: Swine IgG Antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 goat anti-rabbit (p/n 611-143-002) at 1:20,000 for 30 min at RT. Block: (p/n MB-070) for 30 min at RT.